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Thermo Fisher Scientific > Electron Microscopy > Electron Microscopes > 3D Visualization, Analysis and EM Software > Customer Portal > Support Center

VISUALIZATION AND DATA SOLUTIONS

CUSTOMER SUPPORT CENTER

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[Amira 3D Pro, Avizo 3D Pro]

 

Context

When performing segmentation and analysis, you may want to determine exactly how many pixels or voxels belong to a specific material or label.

This can be done using the Label Analysis module, which is described in more detail below.

 

Prerequisites

An Amira-Avizo Software 3D Pro license is required to perform the following workflow.

 

Process

1. Ensure that you have a label image from a previous segmentation.

2. Create and attach a Label Analysis module to that label image.

3. Do not connect a grayscale/intensity image if your goal is to determine the voxel count per material.

4. Define a new measure using one of the two methods below. See Steps for Adding a New Measure if you are unsure how to proceed.

  • Use the Voxel Count measure, which provides a result equivalent result to Material Statistics (deprecated since version 2025.1)​​​​​​.
  • Define Volume3d/(cx* cy* cz) as a custom measure.

5. Click Apply on the Label Analysis module and review the generated spreadsheet. 

     

Steps for Adding a New Measure

 

1. Add a Label Analysis module and click the ... button next to Measures.

                                                                                                                                

2. Click Create a new measure group. Enter a name for the measure group and click OK.

2.1. Click Custom Measure to add a new measure.

2.2. The Measure Editor opens. In the open editor, enter the desired measure, either VoxelCount or Volume3d/(cx* cy* cz), and click Close.


2.3. Click ">" to add the native measure into the group. You can create a new group or add it to an existing group.

2.4. Click OK to assign the measure group to the Measures port of the Label Analysis module.